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Small, accessory chromosomes found in bacteria that are useful in recombinant DNA procedures are called:


A) plasmids.
B) viroids.
C) centrioles.
D) bacteriophages.

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The genetic recombination of ________ caused massive, widespread epidemics that affected the global human population several times in the 20th century.


A) human and bacterial plasmids
B) bacterial and viral plasmids
C) bird flu viruses and human flu viruses
D) HIV and flu viruses

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Ligase enzymes are used to covalently bind together pieces of DNA. True or False?

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Define the term "biotechnology."

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Biotechnology is any genetic a...

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In nature, DNA recombination occurs but it is random and undirected. True or False?

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At least 80% of the ________ grown in the United States is transgenic.


A) corn (only)
B) cotton (only)
C) soybeans (only)
D) corn and soybeans (only)
E) corn, cotton, and soybeans

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Which of the following is NOT a natural means of DNA recombination?


A) sexual reproduction
B) transformation in bacteria
C) viral infection
D) the polymerase chain reaction

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Which of the following involves the use of human genes?


A) herbicide-resistant corn
B) Bt toxin production by soybeans
C) banana-based vaccine against pathogenic E. coli
D) growth hormonetreated cows
E) insulin production by bacteria

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Provide one example of a medical application of biotechnology.

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Possible answers are genetic s...

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The substances used to cut genes in recombinant DNA research are called:


A) DNA polymerases.
B) RNA polymerases.
C) spliceosomes.
D) replicases.
E) restriction enzymes.

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Restriction enzymes are useful in recombinant DNA studies because they:


A) cut DNA only at specific locations.
B) join the cut ends of small DNA molecules.
C) can reproduce in bacteria.
D) give plasmids antibiotic properties.
E) can separate pieces of DNA and RNA from each other.

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If you cut out a gene using EcoRI and BamHI, what enzyme(s) will you need to insert the gene into another chromosome?


A) EcoRI only
B) BamHI only
C) both EcoRI and BamHI
D) neither of these enzymes

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Which of the following is NOT a valid difference between traditional and modern forms of biotechnology?


A) Modern biotechnology can isolate and manipulate individual genes.
B) Traditional biotechnology is almost always limited to genetic recombination between individuals of one species or two closely related species.
C) Traditional biotechnology cannot produce entirely new genes in one step.
D) Modern forms of biotechnology are slower than traditional forms of biotechnology.

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A recombinant plasmid contains only human and bacterial DNA. True or False?

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In biotechnology research, DNA fragments created by restriction enzyme action are separated from one another via:


A) crossing over.
B) gel electrophoresis.
C) centrifugation.
D) filtering.
E) the polymerase chain reaction.

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Based on this figure, to which of the following DNA sequences will the illustrated DNA probe bind? Based on this figure, to which of the following DNA sequences will the illustrated DNA probe bind?   A) AGATCTATCTATCTAGAT B) AGAAGATAGATAGATGAT C) AGAGATATGATAGATGAT D) AGAGATATCTATCTAGAT


A) AGATCTATCTATCTAGAT
B) AGAAGATAGATAGATGAT
C) AGAGATATGATAGATGAT
D) AGAGATATCTATCTAGAT

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________ are small, circular pieces of DNA that are often used in the process of transformation.

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Specific fragments of DNA on a gel can be visualized using:


A) DNA probes.
B) restriction enzymes.
C) electrophoresis.
D) single tandem repeats (STRs) .
E) a polymerase chain reaction (PCR) .

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Which of the following can be used to detect genetic disorders?


A) restriction fragment length polymorphisms (RFLPs) only
B) arrays of DNA probes only
C) single tandem repeats (STRs) only
D) RFLPs and DNA probe arrays only
E) RFLPs, DNA probe arrays, and STRs

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In recombinant DNA technology, plasmids:


A) are used to insert foreign DNA into bacteria.
B) show restriction enzymes where to cut bacterial DNA.
C) are necessary for cellular respiration in certain plants.
D) are the sites of protein synthesis in bacteria.
E) are isolated from the blood of mammals.

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