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Which of the following statements best describes the role of NAD+/NADH in glycolysis?


A) NADH is used to directly create the phosphoanhydride bonds in ATP.
B) NAD+ accepts electrons.
C) NADH accepts electrons during fermentation.
D) NADH is used primarily in substrate-level phosphorylation.
E) All of the above are correct.

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Aldolase breaks down fructose-1,6-bisphosphate into two trioses that are


A) identical.
B) each doubly phosphorylated.
C) identical and each doubly phosphorylated.
D) different and each doubly phosphorylated.
E) different and each singly phosphorylated.

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Reduction is the addition of ________,which usually is accompanied by addition of protons in a process called ________ in biological systems.

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electrons;...

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Each of the following is a substrate for glycolysis except


A) galactose.
B) mannose.
C) lactose.
D) fructose.
E) All are substrates for glycolysis.

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During the process of glycolysis,which of the following substrates donates electrons to NAD+ and adds Pi to form 1,3-bisphosphoglycerate?


A) 3-phosphoglycerate
B) 2-phosphoglycerate
C) glyceraldehyde-3-phosphate
D) glucose
E) glucose-6-phosphate

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________ is the enzyme that hydrolyzes ________ in dairy products into glucose and galactose.Absence of the enzyme may cause cramps and diarrhea in a condition called ________.

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Lactase; l...

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A temporary oxygen deficit during strenuous exercise causes a muscle to


A) synthesize muscle proteins in response to steroid hormone stimulation.
B) synthesize glycogen.
C) ferment pyruvate to lactate.
D) switch to aerobic glycolysis.
E) synthesize more NAD+.

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During glycolysis,aldolase catalyzes the breakdown of fructose-1,6 bisphosphate to produce


A) phosphoenolpyruvate.
B) dihydroxyacetone phosphate.
C) fructose-6-phosphate.
D) A and B.
E) B and C.

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In the first step of glycolysis,glucose can be phosphorylated to glucose-6-phosphate,because


A) a phosphoanhydride bond has higher energy of hydrolysis than is required for formation of a phosphoester bond.
B) transfer of a phosphate from ATP to glucose is endergonic.
C) the reaction does not require an enzyme.
D) the reaction is highly reversible.
E) All of the above are correct.

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The loss of a(n)________ increases entropy during hydrolysis of ATP.

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Glycolytic enzymes are known to function in each of the following processes except


A) transcriptional regulation.
B) gluconeogenesis.
C) apoptosis regulation.
D) oxygen reduction to form water.
E) coordination of glycolysis and mitochondrial respiration.

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________ organisms can function in an aerobic or anaerobic environment.

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A phosphoanhydride bond


A) is a high-energy bond.
B) has a standard free energy of hydrolysis of −7.3 kcal/mol.
C) is broken by hydrolysis.
D) links two phosphate groups.
E) All of the above are correct.

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Although pyruvate is the end product of glycolysis and no more ATP is generated by fermentation of pyruvate to ethanol,yeast produce ethanol under anaerobic conditions because


A) pyruvate export requires more energy than ethanol export.
B) electrons (and protons) are then added to ethanol to make lactate.
C) transfer of electrons from NADH to pyruvate to make ethanol regenerates NAD+,which is necessary for new rounds of glycolysis to proceed.
D) glycolysis substrate-level phosphorylation changes pyruvate to ethanol.
E) pyruvate to ethanol fermentation decreases deleterious CO2 levels.

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Oxidation reactions in biological systems generally involve


A) addition of electrons and hydrogen ions.
B) loss of electrons and hydrogen ions.
C) addition of electrons and loss of hydrogen ions.
D) loss of electrons and addition of hydrogen ions.
E) loss of electrons and either addition or loss of hydrogen ions.

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The ________ links lactate produced by fermentation in muscle cells with gluconeogenesis in liver cells.

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Consider the three phases of glycolysis: (1) preparation and cleavage (Gly-1 to Gly-5) (2) oxidation (Gly-6 to Gly-7) (3) pyruvate formation (Gly-8 to Gly-10) Which of these phases produces ATP?


A) 1
B) 2
C) 3
D) 1 and 2
E) 2 and 3

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The ATP "high-energy" or "energy-rich" bonds are which of the following types of bond?


A) phosphoester
B) phosphoanhydride
C) hydrogen
D) ionic
E) monophosphate

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Glycolysis is a ________-step reaction sequence that converts glucose into ________ and generates a net total of ________ ATPs.

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________ is the most common coenzyme involved in energy metabolism.

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