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Which of the following observations supports the notion that the gene is simply a set of DNA sequences that are transcribed into a single RNA molecule that encodes a single polypeptide?


A) Alternative splicing-a single gene can yield multiple mRNA and protein products.
B) A single ribosomal RNA transcript can liberate several RNA molecules via further processing.
C) RNAs can be the functional product of a gene without being translated into a protein product.
D) Within a protein coding region, each codon represents a specific amino acid that will be linked to form a polypeptide.
E) Regulatory elements are part of a gene that regulate timing, degree, and specificity of gene expression but are not transcribed.

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Below is a list of steps in the processing of ribosomal RNAs. Please select the choice that lists the steps in the CORRECT sequential order. 1. Methyl groups added to specific bases and the 2'-carbon atom of some ribose sugars 2) Transcription of the rRNA precursors from DNA 3) Cleavage of precursor rRNA 4) Individual rRNA molecules ready for ribosome assembly 5) Trimming of precursor rRNA


A) 3, 1, 2, 5, 4
B) 2, 3, 4, 5, 1
C) 4, 2, 3, 1, 5
D) 1, 3, 5, 4, 1
E) 2, 1, 3, 5, 4

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Anticodons are found in _____ molecules.


A) mRNA
B) tRNA
C) rRNA
D) snRNA
E) miRNA

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Which of the following statements about ribosomes and ribosomal RNA is NOT true?


A) Ribosomes typically contain about 80% of the total cellular RNA.
B) Ribosomal RNA is processed in both prokaryotes and eukaryotes.
C) In eukaryotes, genes for rRNA are usually present within tandem repeats.
D) Each ribosomal RNA component is encoded by a separate gene.
E) In eukaryotes, the rRNA transcripts are processed further by snoRNAs within the nucleus.

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Which of the following elements would NOT be found in an mRNA molecule?


A) protein-coding region
B) 3' untranslated region
C) 5' untranslated region
D) promoter
E) start and stop codons

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If a splice site were mutated so that splicing did not take place, what would be the effect on the amino acid sequence?


A) It would be shorter than normal.
B) It would be longer than normal.
C) It would be the same length but would encode a different protein.
D) It would be unable to fold into its correct structure.
E) It depends on the mutant mRNA sequence.

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Devise and describe a strategy to conduct a pulse-chase experiment to determine if newly transcribed eukaryotic RNA actually moves from the nucleus to the cytoplasm.

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To conduct a pulse-chase experiment to d...

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A microbiologist isolated a mutant strain of E. coli that is extremely susceptible to bacteriophage infection, by a wide range of bacteriophages. Which of the following genes might she explore as a possible site of the mutation that results in this phenotype?


A) A gene that encodes a small nucleolar RNA
B) A gene that encodes a Cas protein
C) A gene that encodes a Piwi-interacting RNA
D) A gene that encodes either a miRNA or a siRNA
E) A gene that encodes an immune RNA

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For a time, a gene was defined as a sequence of DNA that encodes a polypeptide. Critique this outdated notion of what a gene is and propose a more comprehensive definition.

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The traditional definition of a gene as ...

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Devise a strategy to prove that splicing occurs in the nucleus.

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To prove that splicing occurs in the nuc...

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The 5' cap on an mRNA is important for all the processes listed below except for the _____ of an mRNA molecule.


A) transcription
B) intron removal
C) stability
D) initiation of translation
E) ribosomal interaction

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Below is a list of steps of eukaryotic pre-mRNA processing. Please select the choice that lists the steps in the CORRECT sequential order. 1. Recognition and binding the 3' AAUAAA sequence by specific protein factors 2) Cleavage at the poly(A) site 3) Addition of the 5' cap 4) Export to the cytoplasm 5) Addition of the poly(A) tail


A) 3, 1, 2, 5, 4
B) 2, 3, 4, 5, 1
C) 4, 2, 3, 1, 5
D) 1, 3, 5, 4, 1
E) 5, 4, 1, 3, 2

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Which of the following regulatory RNA types is different from the rest in terms of its length?


A) siRNA
B) crRNA
C) miRNA
D) piRNA
E) lncRNA

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Explain how RNA editing violates a general principle of molecular genetics.

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RNA editing violates a general principle...

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The calcitonin gene can encode either the hormone calcitonin or a protein called calcitonin-gene-related peptide depending on which 3' cleavage site is used. In the thyroid gland, cleavage and polyadenylation occur after the fourth exon leading to calcitonin production. However, in the brain, the exact same transcript is cleaved after the sixth exon yielding calcitonin-gene-related peptide. This is an example of:


A) multiple capping.
B) alternative RNA processing.
C) polyadenylation.
D) environmental influence.
E) mutation.

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Which of the following intron types requires spliceosomes for removal?


A) group I intron
B) group II intron
C) group III intron
D) nuclear pre-mRNA
E) tRNA

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To which part on a tRNA would an amino acid attach during tRNA charging?


A) 3' acceptor arm
B) anticodon arm
C) T Ψ\Psi C arm
D) DHU arm
E) extra arm

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siRNAs and miRNAs function in which of the following processes?


A) transcription
B) translation
C) RNA interference
D) RNA editing
E) RNA splicing

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Critique the idea that the information needed to encode a particular polypeptide always resides within a single gene.

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The idea that the information needed to ...

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Which of the following nitrogenous bases is frequently modified enzymatically to become a rare type of nitrogenous base in tRNA?


A) adenine
B) uracil
C) thymine
D) cytosine
E) guanine

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