Question 1

Which of the following is used to separate cellular proteins for analysis?

Accepted Answer

A)   Western blots
B)   Subtractive cDNA libraries
C)   Two-dimensional gel electrophoresis
D)   Pulsed field gel electrophoresis
E)   None of the answers are correct

Question 2

The use of mathematics to understand the information within genetic sequences is called mathematical
genomics.

Accepted Answer

A) True 
 B)False

Question 3

A cluster analysis is used when reading a DNA microarray to identify genes that appear to be
expressed together.

Accepted Answer

A) True 
 B)False

Question 4

Which of the following procedures is used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Accepted Answer

A)   Subtractive cDNA library
B)   Treatment with reverse transcriptase to isolate the DNA of interest
C)   Southern blotting
D)   Mass spectrometry

Question 5

Which of the following would be used with a protein microarray to establish protein expression?

Accepted Answer

A)   cDNA
B)   mRNA
C)   Plasmid vectors
D)   Antibodies
E)   None of the answers are correct

Question 6

cDNA uses chromosomal DNA as its template.

Accepted Answer

A) True 
 B)False

Question 7

To identify novel DNA binding sites for a protein, it would be best to use:

Accepted Answer

A)   BLAST
B)   YACs
C)   Chromatin immunoprecipitation
D)   ChIP on chip
E)   Time shift experiments

Question 8

Proteome databases are collections of information on the genes of a specific species.

Accepted Answer

A) True 
 B)False

Question 9

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by .

Accepted Answer

A)   Net charge
B)   Molecular weight
C)   Abundance
D)   None of the answers are correct

Question 10

A functional protein microarray can be used to establish the function or substrates for a given group of
proteins.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following terms best describes two homologous genes found in different species?

Accepted Answer

A)   Homologues
B)   Paralogs
C)   Gene family
D)   Orthologs
E)   None of the answers are correct

Question 12

Genes are to the genome as are to the proteome.

Accepted Answer

A)   carbohydrates
B)   proteins
C)   DNA
D)   prions

Question 13

In the screening of a DNA microarray, detection of a fluorescent signal from one of the genes indicates which of the following?

Accepted Answer

A)   The probe hybridized to that particular gene
B)   The probe did not hybridize to that particular gene
C)   The experiment was not conducted correctly
D)   None of the answers are correct

Question 14

The most common mechanism by which the proteome varies from the genome is

Accepted Answer

A)   Alternative splicing
B)   Posttranslational covalent modification
C)   RNA editing
D)   None of the answers are correct

Question 15

Two genes that share a common ancestry are said to have homology.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following areas of genetics applies mathematical principles to genetic sequences in order to better understand the genetic information?

Accepted Answer

A)   Structural genomics
B)   Functional genomics
C)   Proteomics
D)   Bioinformatics

Question 17

can be used to determine the amino acid sequence of a protein or peptide.

Accepted Answer

A)   X-ray crystallography
B)   nuclear magnetic resonance
C)   mass spectrometry
D)   Chip assay

Question 18

DNA microarray data may be used to determine the relationship between gene expression and protein abundance, however, these results must be confirmed by blot analysis.

Accepted Answer

A)   Western
B)   Eastern
C)   Northern
D)   Southern

Question 19

What is the purpose of sodium dodecyl sulfate (SDS)  in a two-dimensional gel electrophoresis experiment?

Accepted Answer

A)   Allows proteins to be separated by charge only
B)   Allows proteins to be separated by size only
C)   Neither of the above

Question 20

What information would a subtractive cDNA library provide to a researcher?

Accepted Answer

A)   The total protein content of a cell
B)   The number of introns in the genome
C)   The mRNAs that are produced only under specific conditions
D)   The total genes in an organism

Exam 21: Genomics II: Functional Genomics, Proteomics, and

can be used to determine the amino acid sequence of a protein or peptide.

Correct AnswerverifiedShow Answer

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by .

Correct AnswerverifiedShow Answer

What is the purpose of sodium dodecyl sulfate (SDS) in a two-dimensional gel electrophoresis experiment?

Correct AnswerverifiedShow Answer

Which of the following areas of genetics applies mathematical principles to genetic sequences in order to better understand the genetic information?

Correct AnswerverifiedShow Answer

cDNA uses chromosomal DNA as its template.

Correct AnswerverifiedShow Answer

Which of the following procedures is used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Correct AnswerverifiedShow Answer

DNA microarray data may be used to determine the relationship between gene expression and protein abundance, however, these results must be confirmed by blot analysis.

Correct AnswerverifiedShow Answer

Which of the following would be used with a protein microarray to establish protein expression?

Correct AnswerverifiedShow Answer

In the screening of a DNA microarray, detection of a fluorescent signal from one of the genes indicates which of the following?

Correct AnswerverifiedShow Answer

Proteome databases are collections of information on the genes of a specific species.

Correct AnswerverifiedShow Answer

Which of the following is used to separate cellular proteins for analysis?

Correct AnswerverifiedShow Answer

What information would a subtractive cDNA library provide to a researcher?

Correct AnswerverifiedShow Answer

A cluster analysis is used when reading a DNA microarray to identify genes that appear to be expressed together.

Correct AnswerverifiedShow Answer

Two genes that share a common ancestry are said to have homology.

Correct AnswerverifiedShow Answer

Genes are to the genome as are to the proteome.

Correct AnswerverifiedShow Answer

To identify novel DNA binding sites for a protein, it would be best to use:

Correct AnswerverifiedShow Answer

A functional protein microarray can be used to establish the function or substrates for a given group of proteins.

Correct AnswerverifiedShow Answer

The most common mechanism by which the proteome varies from the genome is

Correct AnswerverifiedShow Answer

The use of mathematics to understand the information within genetic sequences is called mathematical genomics.

Correct AnswerverifiedShow Answer

Which of the following terms best describes two homologous genes found in different species?

Correct AnswerverifiedShow Answer

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