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Exam 4: Fundamentals of Molecular Biology

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Question 31

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Proteins are synthesized from RNA templates through a process called _______.

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Question 32

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Consider Mendel's experiments on the genetics of peas. What is the probability that two green parent pea plants would also have green offspring?

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The probability is 100%. All w...

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Question 33

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In the study of molecular biology one frequently hears references to the "central dogma" of molecular biology. What is meant by this term?

To clone genes, a plasmid vector should contain all of the following except

Which technique is used to inactivate a gene by altering the DNA that encodes it?

Gregor Mendel discovered the principles of genetics in his early experiments on

Eukaryotic genes expressed in bacteria produce products that differ from the eukaryotic protein in that they do not have _______ modification.

The DNA made from an mRNA is called _______.

Reverse transcriptase is an enzyme that catalyzes _______ synthesis.

In addition to mRNA, the two types of RNA necessary for protein synthesis are _______ and _______.

Consider this statement: An increase in the levels of a protein within a cell indicates that expression of the gene encoding it was also increased. Why is the statement not necessarily true?

SDS-polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins by

What is the complementary sequence to the following bases 5?-TCAAGG-3?

The first enzymatic step in making a cDNA uses

Taq polymerase is used in the polymerase chain reaction because it

A common method of DNA sequencing is based on the premature termination of DNA synthesis that accompanies the use of dideoxynucleotides in the PCR reactions. Why does elongation of a growing strand cease when a dideoxynucleotide is incorporated?

Most eukaryotic cells transfected with DNA using liposomes will only express the introduced genes for several days because

DNA was identified as the genetic material in experiments done by

Embryonic stem cells introduced into early mouse embryos can give rise to cells in all of the tissues of the mouse, including germ cells.

The CRISPR/Cas system has revolutionized the efficiency with which scientists can mutate a gene in vivo to investigate the functional role of the protein it encodes. Which component is not essential in the CRISPR/Cas system?