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In a stained preparation of epithelial cells from a female mammal, the highly condensed chromosome observable in interphase nuclei is the inactive X chromosome, or _______.

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DNA in eukaryotes is wrapped around special proteins to form structures that look like beads on a string. These structures are called _______.

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Transcription factors


A) have a particular motif that allows them to interact with mRNA.
B) assemble at the promoter to help translation begin.
C) mark particular proteins for degradation.
D) interact with RNA polymerase to initiate transcription.
E) help stabilize the mRNAs in the cytoplasm.

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Transcriptional regulation in prokaryotes can occur by


A) a repressor binding an operator and preventing transcription.
B) an activator binding upstream from a promoter and positively affecting transcription.
C) different promoter sequences binding RNA polymerase more tightly, resulting in more effective transcriptional initiation.
D) the control of promoter efficiency.
E) All of the above

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The site on the operon DNA where a repressor binds is called the _______ sequence.

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Transcription factors are


A) RNA sequences that bind to RNA polymerase.
B) DNA sequences that regulate transcription.
C) proteins that bind to the DNA promoter sequence.
D) polysaccharides that bind to the transcripts.
E) factors that bind to enhancers.

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Bacteriophage DNA that is stably integrated into a bacterial chromosome is called a _______.

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Which of the following statements about histone modifications is false?


A) They cause some genes to be transcriptionally activated.
B) They can result in the repression of gene transcription.
C) They are inherited from parental cells in a Mendelian fashion.
D) They cause Barr bodies to form.
E) All of the above are false.

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Imagine that the TATA box for gene X becomes highly methylated. How will this affect the expression of gene X?


A) There will be no effect.
B) Gene X will be transcribed but not translated.
C) Gene X will be transcribed if the transcription factors receive the appropriate environmental signal.
D) Gene X will not be transcribed or translated.
E) Gene X will be transcribed if the histones become acetylated.

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When the operator is unbound, the binding of the CRP-cAMP complex _______ the binding of RNA polymerase at the promoter.


A) prevents
B) decreases
C) increases
D) blocks
E) All of the above are sometimes true, depending on the concentration of lactose.

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Genes that specify the primary structure of a protein are called _______ genes.

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The term "lysogeny" refers to


A) the stable integration of bacteriophage DNA into the bacterial chromosome.
B) the excision of bacteriophage DNA from the bacterial chromosome.
C) the lysing of a bacterium by a bacteriophage.
D) mutation induced by a bacteriophage.
E) exchange of genetic material between a bacteriophage and a bacterium.

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Which of the following statements about β\beta -globin production is true?


A) A translational repressor protein binds to the β\beta -globin and prevents ribosomes from attaching.
B) Alternative splicing of pre-mRNA results in the production of several different β\beta -globins.
C) Transcription of the β\beta -globin gene is determined by transcription factors, regulators, enhancers, activators, silencers, and repressors.
D) Ubiquitin forms a complex with the β\beta -globin that causes its premature breakdown.
E) The 5´ guanosine cap added to the mRNA is the location where RNA polymerase initiates transcription.

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The transfer of genes by a bacteriophage vector characterizes which type of gene transfer in bacteria?


A) Transformation
B) Conjugation
C) Transduction
D) Transference
E) None of the above

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In eukaryotic cells, promoters are


A) transcribed.
B) transcribed and translated.
C) neither transcribed nor translated.
D) transcribed and then removed.
E) sequences of RNA that are spliced out.

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Expression of some eukaryotic genes can be regulated by translational control. One advantage of translational control is that it


A) provides a means for rapid change in protein concentrations.
B) prevents synthesis of excess RNA.
C) directs proteins to their proper subcellular location.
D) occurs only in zygotes.
E) degrades proteins that are no longer needed.

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Research on E. coli indicates that


A) lactose is the preferred energy source.
B) glucose is the preferred energy source.
C) lactose and glucose are used equally as an energy source.
D) β\beta -galactosidase is required for glucose metabolism.
E) three different proteins are synthesized when glucose is present.

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In eukaryotic cells, a negative regulator or silencer


A) is made of DNA.
B) binds to the enhancer region to block transcription.
C) is located both upstream and downstream from the promoter.
D) binds to the operator to block RNA polymerase.
E) binds to a repressor protein to reduce transcription rates.

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Alternative splicing helps to explain


A) the small number of genes in the human genome.
B) the differences in complexity among organisms.
C) why there are more mRNAs than human genes.
D) the great variety in proteins.
E) All of the above

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A _______ consists of complementary DNA transcribed from an RNA genome.

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